Seven new 2,6-bis((1-phenyl-3-aryl-1H-pyrazol-4-yl)methylene)cyclohexanones (3a-g) were synthesized from cyclohexanone and 4-formylpyrazole aldehyde, via Claisen-Schmidt condensation. The structures of newly synthesized compounds were confirmed by IR, 1H NMR, mass and elemental analyses. All the seven compounds were tested in vitro for their antibacterial activity against two gram positive bacteria namely Staphylococcus aureus and Bacillus subtilis and two gram negative bacteria Escherichia coli and Pseudomonas aeruginosa. The compounds 3b, 3c, 4f, 3g displayed good antibacterial activity when compared with commercially available antibiotic, ciprofloxacin. These compounds also were screened for their antifungal activity against two ear pathogenic fungi, namely Aspergillus niger and A. flavus. The compounds 3c, 3d, 3f, 3g exhibited good antifungal activity when compared with commercially available antifungal, fluconazole.