Thiazole derivatives are known to have wide spectrum of antibacterial activity. So, N-(substituted benzoyl) morpholine/piperidine/N-methyl piperazine carbothioamide (3) were prepared by condensing substituted benzoyl isothiocyanate (2) with different secondary heterocyclic amines, like morpholine/piperidine/N-methyl piperazine. Nsubstituted Carbothioamide (3) on treatment with 2, 5-dimethoxyphenacyl bromide (4) affords 2, 4, 5-trisubstituted thiazoles (5a-h).The newly synthesized compounds were characterized by IR, 1H NMR and Mass spectral studies. The newly synthesized thiazole derivatives were screened for in vitro anti bacterial activity against Escherichia coli, Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Bacillus cereus, Pseudomonas aeruginosa bacterial strains by paper disc diffusion method. All the compounds show moderate to good anti bacterial activity. Among the tested compounds (5f) thiazole carrying 2-piperidino, 4-phenyl, 4-nitrobenzoyl is showing good activity against all the tested species.