The synthesis, characterization and spectroscopic s tudies of new N-substituted pthalimide analogues 2 – 5 with antibacterial activity were described. The compound s were synthesized using phthalic anhydride with va rious amines (aminoethanol , aminopropanol,amino ethyl mo rphline, amino ethyl morphline iodide)in reflux syn thesizer. The purity of the synthesized products were monitor ed by using TLC in an appropriate developing system . The structures of the new synthesized compounds were co nfirmed byusing physical and spectral analysis. Ant ibacterial activity of the synthesized derivatives 2-5 were done in comparison with Phenol as standard com pound. All the selected compounds 2-5 showed very good activity against staphylococcus a ureus(S.aureus), Escherichia coli ( E. Coli ) when compared with Phenol.