Research on 1,3,4-oxadiazole and their synthetic analogs have revealed a variety of pharmacological activities including anti-microbial, antitubercular, anticancer, anti-HIV, antioxidant, analgesic, anti-inflammatory and anticonvulsant activities. A series of 1,3,4- oxadiazole derivatives were prepared from isonicotinamide and hydrazide hydrate, characterized by means of IR, 1H-NMR and mass spectral data. The synthesized compounds were subjected to in-vitro antibacterial activity against Staphylococcus aureus (MTCC-87), Escherichia coli (MTCC-40), Staphylococcus epidermidis (MTCC- 2639), Pseudomonas aeruginosa (MTCC-424) and Proteus vulgaris (MTCC-426) by cup plate method. The analgesic activity was performed by acetic acid induced writhing in mice. Some of the tested compounds exhibited promising biological activities.