Ni(II) and Cu(II) complexes of Pyridoxal Semicarbazone (PLSC) have been synthesized and characterized by elemental analyses, molar conductance and magnetic susceptibility measurements, thermal analysis, ESI-MS, IR and electronic spectral studies. DNAbinding properties of these metal complexes with CT-DNA in a potassium phosphate buffer (pH 7.2) were investigated using UV-Vis absorption spectroscopy, fluorescence spectroscopy and viscosity measurements. Experimental studies suggest good DNA binding ability of these metal complexes through intercalation. The cleavage of plasmid pBR322 DNA without any additives was monitored by gel electrophoresis and these complexes exhibited hydrolytic cleavage of plasmid DNA. The in vitro antibacterial activity of the synthesized complexes has been tested against gram positive and gram negative bacteria.