One pot synthesis of novel quinoline derivatives using aniline, benzaldehyde and its derivatives with acetone in hydrochloric acid as a medium is reported in this article. The Microwave Assisted Organic Synthesis (MAOS) approach was applied to synthesize a series of 2-(4-substituted phenyl)-methyl quinoline derivatives. The structures of the newly synthesized compounds were confirmed by modern analytical techniques like IR, NMR and MS. The synthesized derivatives were tested for their antibacterial activities against standard. Few of the derivatives have been found to possess significant antibacterial activities and can have potential applications in pharmacological science.