Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Target identification and validation for diabetic nephropathy using molecular docking studies

Author(s): Ramanjaneyulu M.*, K. Ananth Kumar, M. Shubash Kumar, Shivakanth Reddy, Divyadhatri Kara, Pravallika Mukthala, Rohith Raj and Chandaka Madhu

Diabetic nephropathy is one of the chronic complications of diabetes mellitus. Inflammatory mediators are believed to play a vital role as predictors of low grade systemic inflammation in diabetic nephropathy. Drug designing, one of the hottest topics have found its new pathway to create a history in the field of medical science. The lead compound analysis starts with CADD, assisting to identify and to optimize the right compound. The technique helps in generating a suitable compound specific to the disease; thereby an effective treatment is achieved. Molecular modeling method has been used for modeling a new molecule for Diabetic nephropathy using Lisinopril , a drug that’s already designed. This drug is drawn using hyperchem, and its R group is modified by replacing different functional groups like OH, Br, CH2CH3, CH3, Cl, F, H, and NH2 , etc in its place and docked by using gold software.. The molecules designed as such are optimized using different algorithms and their affinity is checked with protein. The binding free energy of the protein is calculated by performing docking process. The molecule with minimum binding energy will have the maximum binding affinity. The binding free energy is calculated by the formula Z = Sum of the energy of optimized ligand devoid of solvation parameters and the energy of the protein - ligand optimization. The binding free energy of the designed molecules is obtained by eliminating the energy of the main molecule i.e. Lisinopril .From the results obtained it’s clear that ligand 1 & 5 ( -3.65 & -2.73 ) for Diabetic nephropathy have the maximum binding affinity. So these molecules are determined as the best lead molecules targeting computationally.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
 
Erkek öyle sert ve acımaz sikiyor ki bayan çaresiz bir şekilde tırtıl misali sikiş yataktan aşağı doğru kayıyor fakat hem adam hemde kadın aşırı mobil porno derecede zevk alıyorlar Uzun zamandır karısı ile ilgili planları olan türk porno adam onu başkaları sikerken hayal ediyor bu durumu karısından sikiş izle gizliyordu fakat bir gün adamın moralinin bozuk olduğunu anlayan karısı sikiş ona ne olduğunu sordu ve adamda ona her şeyi anlatmıştı karısı bu porno resim durumdan Büyük zenci yarağı sevenler için özel olarak eklenmiş videomuz türk porno kalın ve damarlı zenci siki arayıp bulamayanlar için özel olarak sikiş izle yayınlanmıştır filmde ise sarışın zengin sürtük büyük mobil sikiş kalın damarlı alet sevdiğini bir çok kez dile getirmiş