Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

The antihyperpigmentation activity test in vitro and the gel formulation’s development from ethanol extract of pine bark as a new natural product

Author(s): Fifi Harmely, Witta Widiasari, Chris Deviarny and Salman Umar

The pine bark has a high antioxidant activity by protecting skin from sunlight’s radiation than can induced the hyperpigmentation abnormality. The antihyperpigmentation activity from aethanol extract of pine bark gel showed by IC50 value of this ethanol extract of pine bark to tirosinase enzyme activity. In this research we have been made the gel formulation from ethanol extract of pine bark by various concentration : 1.0%, 2.0% and 3.0%. Based on the evaluation of the gel formulation we concluded that ethanol extract from pine bark can be formulated as gel formulation and has been fulfill the assential of pharmaceutical formulation. From the three gel formulation we found that those three formulation has characterictic such as the stability for eight week storage time either in cold temperature (50 C) or room temperature (150 C), organoleptically, homogenity and pH. The result of this research, the IC50 value from aethanol extract of pine bark gel is 229.14 μg/ml and the standard M® IC50 value is 183.43μg/ml. This value shows that the antihyperpigmentation activity from aethanol extract of pine bark gel is lower than the standard M®. But a single antihyperpigmentation activity from ethanol extract of pine bark it self has IC50 value 131.08 μg/ml.


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