Anxiety is a common disorder that a lot of people suffer from it and accompanies physiologic symptoms such as tachycardia, perspiration disorders, lack of sense, and sometimes paralysis of limbs etc. This study was performed to compare the anxiolytic effects of herbal tea bag of Lavandula angustifolia versus Valeriana officinalis and these effects were evaluated and compared with the control group. In order to do this, 18 male rats weighing 150 to 200 grams were applied. The rats were divided into three groups including control, valerian (treated group by Herbal tea of Valeriana officinalis ) and valender (treated group by Herbal tea of Lavandula angustifolia).valerian and lavender groups rats (n = 6/group) had ad libitum access to the tea from Valeriana officinalis 3% (w/v) for valerian group and tea from Lavandula angustifolia 3% (w/v) for lavender group , for a period of 24 hours before the test. Then, the behaviour of rats was tested to sedative (locomotor activity) and anxiolytic (elevated plus maze) activity. All the data were given as means±S.E.M. Data were analysed by one-way ANOVA following by Tukey test. The study indicated that anxiolytic effect of Lavandula angustifolia herbal tea is greater than herbal tea of Valeriana officinalis on the male rats.