Nowadays, raising broilers plays key role in meeting the protein requirements of human. About 2 to 5 percent of animal’s body weight is made from minerals. Copper is one of the essential elements of animals which is stored in liver. Current research was carried out to study the effect of adding copper sulfate and copper glycine to diet on liver enzymes of broilers. 420 broilers from Ross race (308) were studied. Treatments were control (diet based on corn and soybean) and six experimental groups including 8, 16, and 32 mg of copper glycine and copper sulfate for 42 days. Sample bloods were taken at 21 and 42 days. Copper did not affect AST in experimental groups. ALT amount in 8 and 16 mg groups of copper sulfate and 8 mg of copper glycine was significantly different from control group (p<0.05). ALP amount was significantly different in 8 mg group of copper sulfate and 32 mg of copper glycine (p<0.05). In conclusion, it can be said that copper as an essential element could not affect regulation of liver enzymes especially ALT and AST. But, it had positive effects on ALP.