Using pharmaceutical plants in reducing stress can play key role in preventing damages of heat stress. The goal of this study was investigating the anti-stress effect s of garden sage (Salvia officinalis)in mice. Fifty mature mice were studied in five groups including control, 0, 100, 2 00, and 400 mg/kg weight of body of hydro alcoholic extract in drink water. Mice were under heat stress (36 ± 0.5° C) for four hours a day. At end of period, blo od samples were prepared and liver enzymes AST, ALT, and ALP were s tudied. According to results, ALP was decreased in zero groupswhereas100 mg/kg compensated the reduction (p < 0.05). AST amount was not different in treatment groups. The amount of ALT was decreased in zero group but 2 00 and 400 mg/kg doses compensated the reduction (p < 0.05). On the whole, hydro alcoholic extract of gar den sage could reduce negative effects of heat stre ss on liver enzymes does dependently.