The physico-chemical properties of the biodiesel prepared from Sweet almond seed oil was determined and discussed in accordance with ASTM D6751 and DIN 14214 standards for biodiesel. The influence of the fatty acid composition and functional groups on the fuel related properties was carried out using Gas Chromatography-Mass Spectrometry (GC-MS) technique and Fourier Transform Infrared Radiation (FTIR) spectroscopy analyses respectively. The presence of C-H indicates the presence of properties as pour and cloud point that affect the performance of biodiesel during cold weather engine operations but the biodiesel sample was found to contain more C=C that can cause it to remain in liquid state with tendency of poor storage stability. It was equally observed that the Sweet Almond Seed Oil Methyl Ester (SASOME) is mainly composed of monounsaturated fatty acid and expected to possess low values for density, viscosity, heating value and thermal efficiency with average cetane number. This would subsequently be translated into low HC, CO and smoke emissions.