Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Abstract

Total phenolic content, cytotoxic and antioxidant activities of Quisqualis indica (Linn.) growing in Egypt

Author(s): Ahmed A. A. Abd El-Rahman1, Ibrahim M. Abd El-Aleem1, Laila A. Refahy2 and Maha A. El-Shazly2

The aim of this study is to evaluate the antioxidant, cytotoxic activities and total phenolic content (TPC) of different fractions of Quisqualis indica(Linn.) leaves. The antioxidant activities were carried out quantitatively using 1,1'- diphenyl-2-picraylhydrazyl free radical (DPPH), phosphomolybdenum and reducing power assays, also qualitatively via dot-blot DPPH staining assay. Also, the cytotoxic activity was evaluated via brine shrimp lethality test (BSLT) and MTT assay against liver carcinoma cell line (HepG-2).The antioxidant activity results against DPPH radical (SC50) were ranged from 24.38 to 72.10 μg/mlwith respect to ascorbic acid (SC50 = 7.45μg/ml), among all tested fractions EtOAc is the most active, furthermore the total antioxidant capacity results were ranged from 250.76 to 16.17 (mg AAE /g dry ext.),moreover at concentration 200 μg/ml the reducing power results (OD values) were arranged as; n-BuOH 0.680 ?? defatted 90% MeOH (0.465) ?? EtOAc (0.405) ?? 90% MeOH (0.225) ?? H2O (0.90), in comparison with ascorbic acid (0.985). On the other hand, the cytotoxic activities were arranged as; n-BuOH (LC50= 100), 90% MeOH (LC50= 150), EtOAc (LC50= 170), pet. ether (LC50= 170) and defatted 90% MeOH (LC50= 440) μg/ml.Finally, the cytotoxic results against (HepG-2) revealed that CH2Cl2 & n-BuOH are the most strong cytotoxic fractions (IC50= 11.9, 17.9μg/ml respectively) against Doxorubicin (IC50 = 4 μg/ml). In conclusion, Quisqualis indica consider as a good source of naturally occurring antioxidant and cytotoxic agents.


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