Simple spectrophotometric methods are described for the determination of Thiocolchicoside in bulk and in pharmaceutical formulation. Thiocolchicoside shows the maximum absorbance (λmax) at 259.8 nm in water and was utilised for its determination, Method A. Method B utilises Area under Curve (AUC) in wavelength range of 269.8-249.8 nm. Drug was found to follow the Beer-Lambert’s law in the concentration range of 5-50μg/ml for both the methods. The limit of detection and limit of quantification was found to be 1.49 μg mL-1& 4.51μg mL-1 for Method A and 1.08 μg mL-1 & 3.27 μg mL-1 for Method B. The proposed method was successfully applied to the determination of Thiocolchicoside in pharmaceutical formulations without any interference from excipients. Results of the analysis were validated statistically and found to be satisfactory.