Quantitative estimation of Trapidil and its formulation by UV spectroscopy methods was developed. The Shimadzu UV 1700 double beam UVvisible spectrophotometer and Jasco v-63 UV-spectrophotometer was used for the determination of Trapidil in the formulation. In the UV method, trapidil was quantified at 304 nm and 225.4 nm by A1%,1 cm value and by comparison with standard at both the wavelength while for zero order derivative 293.8 nm and 316.4 nm for first order derivative in 0.1N NaOH were selected. Recovery studies of 98.8-101.14%, percentage relative standard deviation (RSD less than 2%) and correlation coefficient (linearity range) that developed methods were accurate and precise. This method can be employed for the routine analysis of formulation containing trapidil.