Chiral Liquid Chromatographic (CLC) method has been established and authenticated for the enantiomeric resolution of epichlorohydrin. Chiral resolution was achieved on Chiralpak-IA immobilized amylose based stationary phase using an eluent consisting of n-hexane:2-propanol (100:2 v/v) and flowing through column at rate of 1.0 ml/min. The column effluent was examined by using UV detector at 205 nm. Resolution between S-(+)-Epichlorohydrin (SE) and R-(-)-Epichlorohydrin (RE) was found to be ≥ 5. The method was authenticated for the quantification of RE in SE. The detector response for RE was proportionate over the concentrations range of 3-40 μg/ml. The Limit of Detection (LOD) and Limit of Quantification (LOQ) for RE were 0.95 μg/ml and 3.2 μg/ml respectively. Average recovery of the RE was in the range of 96.20% to 101.99%. Analytical solutions of both enantiomers in diluent were found to be stable for a day. The proposed method was found to be precise, accurate and robust for quantitative analysis of RE in SE.
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