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Validated chromatographical methods for the simultaneous estimation of antihypertensive drugs in multicomponent formulations | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X

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Abstract

Validated chromatographical methods for the simultaneous estimation of antihypertensive drugs in multicomponent formulations

Author(s): N. Delhiraj and S. Anbazhagan

Two new, rapid, precise, accurate and specific chromatographic methods for the simultaneous determination of Telmisartan,Amlodipine besylate and Hydrochlorothiazide in combined pharmaceutical dosage forms. The first method based on reverse phase liquid chromatography by using Qualisil BDS C18 column (250 mm X 4.6 i.d., 5 μm). Mobile phase consists of 1.0 ml of triethylamine in one litre water and the pH was adjusted to 2.5 with orthophosphoric acid and Acetonitrile (60:40) with a flow rate of 1ml/min, with a detection wavelength of 281nm.The second method involved silica gel 60F254 high performance thin layer chromatography and densitometric detection at 281 nm using chloroform: methanol:formic acid(85:15:5) as the mobile phase.


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