A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of irbesartan and hydrochlorothiazide in combined dosage forms. The stationary phase used was precoated silica gel 60F. The mobile phase used was a mixture of acetonitrile: ethyl acetate (8:2 v/v). The detection of spots was carried out at 260 nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 100 to 600 ng/spot for irbesartan and 50 to 250 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for the irbesartan were found to be 22.19 and 73.98 ng/spot respectively and for hydrochlorothiazide 12.42 and 41.394 ng/spot respectively. The proposed method can be successfully used to determine the drug content of marketed formulation.
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