Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Abstract

Validation of an UPLC-MS/MS method for simultaneous Quantification of two Selective Serotonin and nor Epinephrine Reuptake Inhibitors and one Selective Serotonin Reuptake Inhibitor in human plasma

Author(s): R. Udhaya, Petla. Y. Naidu, A. Rajasehkara Reddy, T. V. Vidya Sagar

A simple and sensitive Ultra Performance Liquid chromatography-mass spectrometry (UPLC-MS/MS) method was developed and validated for simultaneous estimation of two Selective Serotonin and Nor Epinephrine Reuptake Inhibitors (Duloxetine &Venlafaxine) and one Selective Serotonin Reuptake Inhibitor (Paroxetine) in human plasma using Sertraline as an internal standard. The above mentioned compounds and the internal standard were extracted from 0.5 ml plasma by solid phase extraction method. The analytical separation was carried out in a reverse liquid chromatography at acidic pH 3.9 at isocratic mode. All analytes were monitored in multiple reaction monitoring mode using the respective [M+H] +ions, m/z 298.06/153.90 for Duloxetine, 279.20/57.75 for Venlafaxine,330.61/162.83 for Paroxetine and m/z 306.14/158.71 for the internal standard. The proposed method was validated with linear range of 0.5 -100ng/ml for Duloxetine, Venlafaxine and Paroxetine. The %R.S.D of intraday and inter-day assay was lower than 15%. For its sensitivity and reliability, the proposed method is particularly suitable for pharmacokinetic studies.


PDF

FIND ARTICLES
SCImago Journal & Country Rank
Recommended Conferences
bornova escort karşıyaka escort osmangazi escort buca escort anne porno fetiş porno anal porno bartın escort burdur escort eskişehir escort escort izmir bursa escort porno escort bayan