Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

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Abstract

Validation of Analysis Method α-Mangostin Compound on Skin Fruit Extract Kandis Acid (Garcinia iowar Rob. Ex Choisy) Method HPLC

Author(s): Roslinda Rasyid, Fitra Fauziah, Rahmadonil Akbar

This study uses a method of High-Performance Liquid Chromatography (HPLC) for the analysis of α-mangosteen in the extract Kandis. Analyses were performed with a flow rate of 1 ml/min using Phenomenal C18 column (150 × 4.6 mm) detector used was a UV detector at a wavelength of 240.5 nm. The mobile phase used was acetonitrile: 0.1% phosphoric acid in the ratio (85: 15) v/v. The regression equation of the calibration curve with the concentration of 1, 3, 5, 7 and 9 mg/ml, y=66474x-4456 validation of methods of analysis have been qualified parameter validation analysis, the value of accuracy of 98.62%, 97.90%, 95, 75% precision with 1.27%, 0.7%, 0.3% linearity with correlation Toe Fien 0.9998, the Limit of Detection (LOD) and Limit of Quantification (LOQ) limits respectively 0.22 mg/ml and 0.73 mg/ml. Levels of acid α-mangosteen in lands gained 0.05%.


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