The six coordinated Ruthenium complex of formula [Ru(phen)2(dmso)Cl]+Cl- (cis-chlorodimethylsulphoxide-Sbis( 1,10-phenanthroline) ruthenium (II) chloride) has been prepared, and characterized structure on the basis of spectroscopic data, such as FT-IR, 1H NMR and UV-Vis spectroscopy. The molecular structure of the complex determined by X-ray diffraction studies is found to be octahedral. Also the bond lengths, bond angles and dihedral angles are found complementary to octahedral structure. Further studies on the binding of this complex with Calf Thymus DNA (CT-DNA) have been carried out by using UV-visible spectroscopy, electrochemical and gel electrophoresis studies. The evidences of CT-DNA binding with this complex have been indicated from these studies. The anticancer activity of this compound against Dalton’s lymphoma (in vitro and in vivo) is found promising.