A Novel Method for the Estimation of Budesonide in Human Plasma by Using LC-MSMS | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.


A Novel Method for the Estimation of Budesonide in Human Plasma by Using LC-MSMS

Author(s): Raveendra Babu Gudimitla, Lakshmana Rao Atmakuri, Venkateswara Rao Jangala

A novel method for the estimation of Budesonide in human plasma by using LC-MS-MS and the analyte is budesonide and internal standard is levenorgestreol were extracted with the tertbutyl methyl ether: n-hexane (70:30, v/v) from human plasma. The chromatographic severance was attained of the peak using Agilent Zorbax Eclipse XDB-C8, (100 mm × 4.6 mm, 3.5 μm) column with a run time is 2.5 min. Budesonide and levenorgestreol were recorded at the total ion current of their relevant multiple reaction monitoring. The LC-MS-MS system composed an Agilent 1100 infinity combined with an AB Sciex Qtrap® 4000 thermo Finnigan TSQ quantum discovery triple quadruple mass spectrometer. All of the parameters must be validated like selectivity, accuracy, precision, linearity, lower limit of quantification, matrix effect, recovery reached the acceptance criteria under the following of ICH guidelines. Budesonide have checked the various stability studies like short term stability at 25°C, long term stability for 55 days at -70°C, wet extract stability for 54 h, auto sampler stability for 63 h, bench top stability for 14 h and freeze-thaw stability at -60°C. Hence, it can be used for routine drug analysis and bioequivalence studies of budesonide in human plasma samples.

Full-Text PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry