Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Anti-Protozoal Effect of Some Medicinal Oils on Potentially Pathogenic Naegleria Species

Author(s): Mahmoud Afw Gad, Ahmad Zakaria Al-Herrawy, Sabry Abou-Elfath Mahfouz

About 35,000 to 70,000 plants species has been used as medicaments. Nearly 70 percent of the world’s population relies on plants for their primary health care. Different concentrations from some medicinal plant oils (Eucalyptus citratus, Rosmarinus officinalis and Allium sativum) at different contact times were separately applied to cysts and trophozoites of Naegleria sp. (accession Number: KU312284) isolated from Egyptian swimming pool. Naegleria sp. viability was tested by culturing on non-nutrient agar and incubation at 45ºC and by staining with trypan blue vital stain. The results of the present study showed that the minimum amoebicidal concentrations of essential oils from Eucalyptus citratus, Rosmarinus officinalis and Allium sativum that produced complete death of Naegleria sp. trophozoites reached 15% with contact time 90 min, 25% with contact time 60 min, 15% with contact time 60 min, respectively. The minimum amoebicidal concentrations of oils from Eucalyptus citratus, Rosmarinus officinalis and Allium sativum that produced complete death of Naegleria sp. cysts reached 50% with contact time 6 days, 100% with contact time 3 days, 75% with contact time 3 days, respectively. In conclusion, Eucalyptus citratus showed the best concentration for inactivation of Naegleria sp. cysts and trophozoites.


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