In a way to find a new fungal isolate capable of degrading Polyneuclear Aromatic Hydrocarbons (PAHs) present in water, fourteen fungal isolates were recovered from two sites along the river Nile main stream. Screening of the fungal isolates revealed the selection of FX8 which was identified as Aspergillus flavus as the most potent one, further studies optimization studies indicated that maximum degradation was attained at pH 4 and incubation temperature 30°C at 150 rpm. Gas Chromatography-Mass Spectroscopy (GC-MS) analysis of mono aromatic mixture showed that the fungus can degrade efficiently all compounds as rhe share the same chemical structure. High Performance Liquid Chromatography (HPLC) profiles of the residuals of the sixteen most hazardous polyneuclear aromatic compounds revealed the ability of A. flavus to degrade nearly 85.4% of the mixture only after 4 days of incubation under optimum culture conditions. This supports its application in bioremediation programs of hazardous aromatic pollutants.