Chemical speciation of Ca (II), Zn(II)and Mn(II) complexes of L-Proline and L-Valine in 0.0-2.5 % w/v SLS-water mixtures has been studied pH-metrically at a temperature of 303K and at an ionic strength of 0.16 mol L−1. The selection of best fit chemical models is based on statistical parameters and residual analysis. The predominant species detected were ML+, MLH2+ and ML2H+ for Ca(II), Zn(II) and Mn(II). Models containing different numbers of species were refined by using the computer program MINIQUAD75. The appropriateness of the experimental conditions was verified by introducing errors deliberately. The chemical speciation, metal bioavailability and transportation have been explained based on stability constant and distribution diagrams drawn using HYSS HYPERQUAD.
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