Cytotoxic evaluation using Peripheral Blood Mononuclear Cells (PBMCs) was achieved by a novel series of (E)-1-(4-chlorobenzyl)-N'- benzylidene-5-bromo-1H-indole-2-carbohydrazide 6(a-j). Among the series, the compounds 6b, 6c, 6f, 6h and 6j were found to be active compared to other analogues. These compounds showed excellent dose dependent cytotoxicity on PBMCs, whereas, 6j showed reduction in activity. The in silico pharmacokinetic parameter study such as ADME and toxicity of the synthesized compounds were evaluated using ACD/Ilab and [email protected] methods. The synthesized molecules 6a, 6b, 6c, 6f, and 6g are in acceptable range and less toxic in nature.