Lung cancer is one of the most lethal worldwide and has a dismal prognosis. This disease has become important as a public health concern. Conventional radio-chemotherapies cause adverse side effects ranging from gastro-intestinal complications to bone marrow failure. The inadequate availability of efficient and safe treatment for the cancer intends the scientific researchers to focus the phytochemicals derived from medicinal plants. Lindernia madayiparense (Family: Linderniaceae) is widely distributed in the laterite hills, Kerala, India. Petroleum ether, ethyl acetate and ethanol extracts of L. madayiparense were prepared by continuous hot extraction method using Soxhlet apparatus. All the extracts were assessed for their in-vitro cytotoxic effect against Human lung carcinoma cell lines (A-549 cell line) by MTT assay. The maximum cytotoxic effect (90.96%) was observed by ethyl acetate extract of L. madayiparense at a concentration of 1000 μg/ml against the A-549 cell lines followed by pet ether extract and ethanol extract. The CTC50 value for ethyl acetate extract of L. madayiparense was found to be 594.81 μg/ml.