Extraction of the leaf extract of S. angustifolia followed by an extensive column chromatography of n-butanol portion of the extract on silica gel, purification over sephadex LH – 20 and subsequently (PTLC) was conducted using standard chromatographic and spectroscopic protocols. A bioactive constituent (compound S) from the n-butanol portion of the leaf extract of S. angustifolia was isolated. The structure was elucidated mainly by using IR, FTIR and combination of 400MHZ and 100MHZ 1D and 2D NMR techniques. Thus compound S was determined as 2'-O-p-hydroxybenzoyl-6'-O-trans- Caffeoyl-8-epiloganic acid. The Antispasmodic studies of the various partition portion of the leaf extract were conducted using rabbit jejunum. The n-butanol leaf portion of the extract produced a concentration dependent contraction of the rabbit jejunum (0.04mg/ml-0.32mg/ml). The contraction of the leaf extract at (0.032mg/ml) was blocked or antagonized by atropine at (0.1mg/ml). The preliminary phytochemical screening of the partition portion of the leaf extract conducted reveals the presence of Tannins, Glycosides, Flavonoids, Saponin, Steroids, Alkaloids, and Terpenoids.
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