Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Nephroprotective Potentials of Green Tea through MPTP in Gentamicin Induced Nephrotoxicity in Rats

Author(s): Gurfateh Singh, Hardeep Kaur, Deepika Bhatia, Monisha Bansal

Purpose: To investigate the nephroprotective potentials Green tea extracts dose dependently in gentamicin induced experimental nephrotoxicity via Mitochondrial Permeability Transition Pores (MPTP).

Methods: Six groups of rats employed in present study each group comprises 5 animals. Experimental nephrotoxicity induced by administration of gentamicin (80 mg/kg body weight/day, i.p) for six consecutive days. The thiobarbituric acid reactive substances, superoxide dismutase and glutathione levels in the kidney tissues measured for oxidative stress. Nephrotoxicity evaluated by serum creatinine, Blood Urea Nitrogen (BUN) and histopathological examination.

Results: Gentamicin induced nephrotoxicity characterized by a significant increase in BUN, and serum creatinine levels. Moreover oxidative stress was noticed in renal tissue as evidenced by a significant decrease in glutathione level, superoxide dismutase also a significant increase in malondialdehyde levels in gentamicin & atractyloside treated rats. Pre-administration of green tea extract (100 or 200 mg/kg once daily, p.o) for 15 days restored renal functions and attenuated oxidative stress.

Conclusion: Green tea extract ameliorates gentamicin induced nephrotoxicity and atractyloside induced opening of MPTP leading oxidative damage by scavenging oxygen free radicals, decreasing lipid peroxidation and improving intracellular antioxidant defense and may closing of MPTP.


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