The present study was to elucidate the phytochemical content and antioxidant activity (by DPPH and FRAP) of crude leaf extract (CLE), crude seeds extract (CSE) and alkaloid seeds extract (ASE) from Peganum harmala. Phytochemical screening revealed the presence of tannins, sterols and steroids, coumarins, anthocyanins and saponins. DPPH method specifies an IC50 equal to: 112.96 μg/mL, 35.83 μ/mL and 107.91 μg/mL respectively for leaf, seeds and alkaloids extracts. Standard ascorbic acid shows IC50 value of 4.24 μg/ml. Reducting power revealed that all extracts from Peganum present less significant effect and weaker than that of standard ascorbic acid (0.66, 0.30, 0.58 absorbance for seeds, leaf, alkaloids seeds at 800 μg/ml, ascorbic acid 3.82 absorbance at 200 μg/ml). The presence of secondary metabolites in leaves and seeds of P. harmala could be attributed for the antioxidant activity.
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