Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

Abstract

Setting up and Calibration of Simultaneous Dual Color Wide Field Microscope for Single Molecule Imaging

Author(s): Diaa Atta, Aly Okasha and Medhat Ibrahim

Single molecule spectroscopy is considered among the most important spectroscopic and microscopic analytical tools especially that follow the biological reactions. The usage of wide field microscope in single molecule spectroscopy is not something new but what we introduce is a simultaneous dual color imaging wide field microscope setup. The setup alignments tested, and all the species we used to prepare the sample were tested free from unwanted fluorescence. A real single molecule experiment was done using streptavidin stained with Atto655.The system is proved to be able to detect single molecules in two channels exited with different colors at the same time, and it is ready for the experiments.


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