Pharmaceutically active compounds are the emerging water contaminant and recently numerous studies have reported their continuous release in aquatic system posing an adverse impact on living organism on ecology. Simvastatin belongs to the drug class 3-Hydoxy-3-Methyl Glutaryl Coenzyme-A (HMG-CoA) reductase inhibitor. The present study focuses on the efficient loading of simvastatin drug onto the surface of Pleurotus florida biomass (PFB). The isothermal, kinetic and thermodynamic parameters were examined and the results showed that maximum simvastatin uptake was observed at 5.49 mg.g-1 with removal efficiency 72.56%. The maximum sorption capacity calculated from the Langmuir isotherm model was found to be 76.92 mg.g-1 and the separation factor value lies between zero and one indicating favorable isothermal process. The kinetic design was well illustrated by the pseudo second order model. Thermodynamic parameters imply that the adsorption of simvastatin on PFB was an endothermic process and the functionalities responsible for the simvastatin uptake were accomplished by Fourier Transform Infra-Red (FTIR) spectroscopy and Scanning Electron Microscopy (SEM). Results depict the applicability of oyster fungi as economically viable tool for the pharmaceutical enriched effluent treatment.