Performance liquid chromatographic method was developed for estimation of two Anti-hypertensive drugs, Olmesartan medoxomil and Chlorthalidone. The separation of both the drugs was achieved on Phenomenex kinetex XB C-18 column (150 mm X 4.6 mm , 5μ particle size) using mobile phase Acetonitrile : 0.1% TFA Water (45:55v/v). The flow rate was 1 ml/ minute with run time 10 minutes and detection was done at 235 nm. The retention time of Olmesartan medoxomil and for Chlorthalidone was 2.15 min. and 1.71 min. respectively. The linearity of the method was evaluated at a range of 50 to 150 μg/ml and 31.25 to 93.75 μg/ml for Olmesartan medoxomil and Chlorthalidone respectively. The Correlation Coefficient of Olmesartan medoxomil and Chlorthalidone were 0.999 each. Precision studies were carried out and %RSD of peak areas of Olmesartan medoxomil and Chlorthalidone was about 0.051 and 0.077 respectively. The percentage recoveries of both the drugs OLM and CHL from the tablet formulation were 98.27% and 98.33% respectively. The developed method has been statistically validated according to ICH guidelines and found to be simple, cost-effective, linear, precise, and accurate with the prescribed values. Thus this method can successfully employ for simultaneous estimation of both drugs in commercial products.
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