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Synthesis, spectral analysis and bioactivity of new inexpensive and diversity oriented method for the synthesis of various polysubstituted quinolines through the condensation of o-amino aryl carbonyls with ketones containing an active methylene group. We have synthesized twenty Quinoline derivatives were synthesized in moderate to good yields. The structures of all the newly synthesized molecules were assigned by elemental analysis and spectral data. The synthesized compounds were screened for their antibacterial activities strains using Cup plate method.