In search of new chemical nucleases the synthesis of methylmalonohydrazide based hydrazone Schiff base and its colored complexes with transition metal Co(II), Ni(II), Cu(II), Mn(II), and Zn(II) have been synthesized. All synthesized compounds were screened for antibacterial activity against gram (+) and gram (-) bacteria using Oxacillin and Penicillin-G as a reference standard and their DNA photocleavage potential were carried out by using the plasmid DNA via gel electrophoresis method. From experimental results it has been observed that biological activity increases significantly upon coordination. The synthesis of hydrazone Schiff’s base, N,N’-bis(1-(2-Hydroxyphenyl)ethylidene)-2-methylmalonohydrazide (MAPLH2) have been achieved by a condensation reaction between 2-methylmalonohydrzide and 1-(2-Hydroxyphenyl)ethanone. Furthermore, new transition metal complexes were formed by the reaction between novel ligand and different transition metal ions in 1:1 stoichiometric ratio. All the synthesized compounds were characterized on the basis of various spectroscopic techniques like Proton Nuclear Magnetic Resonance (1H-NMR), Ultraviolet (UV)-Visible, elemental analysis, Fourier-transform infrared (FT-IR) and mass spectroscopy. 1H-NMR data of complexes showed the coordination of hydroxy atom with subsequent loss of proton and FT-IR spectral data reveals that the participation of nitrogen atom of ligand in coordination with metal ion.