The accumulating pharmacological importance of drug resistant pathogens has lent auxiliary urgency to new anti-tubercular compound development. In this regard, a novel series of pyrrolo [1,2-a]pyrazine incorporated indolizine derivatives have been synthesized, characterized by using spectral data and screened for in vitro antimycobacterial activity. The anti-tubercular activity of the synthesized compounds (7a-p) was determined by microplate alamar blue assay and the outcomes were screened against Mycobacterium tuberculosis H37Rv strain. Compounds 7a-p exhibited good to potent anti-tubercular activity when compared with the standard first line anti-tuberculosis drugs (ciprofloxacin, pyrazinamide and streptomycin). Some of the tested compounds exhibited highest inhibitory activity at 1.6 μg/ml minimal inhibition concentration.