One of the primary factors shaping the relationships within the organization and employee’s attitude is the Ethical climate governing in the organization that is one of the factors affecting job interestedness. Job interestedness is an integral part of an employee’s character that is defined as a person’s imagine of job and as an important part of working life. The aim of this research is to study the relationship between the Ethical climate and job interestedness. This study is descriptive-analytical study which was examined 71 individuals in Zahedan. To collect the data a three-part questionnaire was used that the first part is related to the demographic features. The second part is standard questionnaire on job interestedness and in third part is used Olson’s questionnaire. Finally, after gathering the data they were analyzed in SPSS 19 software by statistical tests, t-test and variance analysis. The average age of individuals was 28.34 ± 5.05 and 59 personnel were female and 12 personnel were male. The relationship between demographic features and job interestedness was not significant (P>0.05). The relationship between the variables of age, gender with job interestedness was not significant (P>0.05). But between the variables of passing ethics course (P=0.001), ethnicity (P=0.008) and the hospital of working place (P=0.01) and the Ethical climate there was significant relationship. Generally, the average score of Ethical climate and among the operating rooms’ personnel in the educational hospitals of Zahedan was 93.15 ± 15.17 and the average score of job interestedness was 28.33 ± 7.55 and a significant relationship between the job interestedness and the Ethical climate was not observed. The results indicated that accordingly the personnel perceived the Ethical climate very well, they have lower job interestedness and there was not a significant relationship between the Ethical climate and job interestedness.