Electrochemical and Spectroscopic Evaluation on Interaction of Anticancer Drug Bicalutamide with DNA | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.


Electrochemical and Spectroscopic Evaluation on Interaction of Anticancer Drug Bicalutamide with DNA

Author(s): Umar J Pandit, Gowhar A Naikoo, Gulzar A Khan, Sneha Wankar, Imran Khan, Raj KK and Limaye SN*

Differential Pulse (DP) voltammetry, spectrophotometry, fluorescence emission spectroscopy and docking simulations were employed to investigate the interaction of an anticancer drug Bicalutamide (BIC) with CT-DNA (Calf thymus-DNA). At modified single walled carbon nanotube carbon paste (SWCNT-CP) electrode the anticancer drug BIC produced a well-defined cathodic reduction peak which decreased on addition of CT-DNA. On increasing concentrations of CT-DNA no shift of peak potential of BIC was observed, characteristic of Groove binding mode of interaction. A binding constant (K) of 2.13 × 106 M-1 was determined by DP voltammetric method. In addition, the interaction of BIC with CT-DNA was further examined by spectrophotometric and fluorescent emission techniques. Interestingly, the binding constants (K) obtained through spectrophotometric method and voltammetric technique was in close agreement. However, the groove binding interaction of drug and DNA was also revealed by spectroscopic studies which were supported by theoretical docking studies.

Full-Text PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Google Scholar citation report
Citations : 25868

Der Pharma Chemica received 25868 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons
Der Pharma Chemica- Journals on pharmaceutical chemistry