Stability Indicating Method Development and Validation of Lamivudine, Zidovudine and Nevirapine by Using HPLC | Abstract

Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X


süperbetin giriş

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission Systemof respective journal.

agariookey oyna istanbul okey


Stability Indicating Method Development and Validation of Lamivudine, Zidovudine and Nevirapine by Using HPLC

Author(s): Som Shankar Dubey* and Mahesh Duggirala

A simple, efficient, and robust stability-indicating RP-HPLC method has been developed and validated to measure Lamivudine, Zidovudine and Nevirapine at a single wavelength (268 nm) in order to assay. The samples were eluted in an isocratic method using an inertsil ODS column (4.6 mm × 250 mm with a particle size of 5 μ) with a mobile phase consisting of 10 mM Ammonium acetate buffer (with a pH adjusted to 3.8 using Acetic acid): acetonitrile (60:40, v/v), acetonitrile and water (1:1, v/v) using as diluent, through ambient temperature delivered at a flow rate 1.2 mL/mins. A good linear response was obtained in the range from 15-75 μg/mL, 30-150 μg/mL and 20-100 μg/mL of Lamivudine, Zidovudine and Nevirapine respectively. The LODs for Lamivudine, Zidovudine and Nevirapine were found to be 0.315, 0.405 and 0.600 μg/mL, respectively and the LOQs for Lamivudine, Zidovudine and Nevirapine were 0.945, 1.080 and 2.100 μg/mL respectively. The method was quantitatively evaluated in terms of accuracy (recovery), linearity, precision, selectivity and robustness in accordance with standard guidelines. The method is simple, suitable and conducive for analyzing Lamivudine, Nevirapine and Zidovudine in bulk and in pharmaceutical formulations.

Full-Text PDF

Select your language of interest to view the total content in your interested language

30+ Million Readerbase
SCImago Journal & Country Rank
Recommended Conferences
Google Scholar citation report
Citations : 11575

Der Pharma Chemica received 11575 citations as per Google Scholar report

Der Pharma Chemica peer review process verified at publons

rokettube brazzers

Turkish dental care is known to be affordable compared to other countries. If you want an affordible dentist Turkey, check out periodonta. It is heavily recommended for dental patients visiting Turkey