Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X


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Mahesh Duggirala

Department of Chemistry, Gitam Institute of Technology, Gitam University, Visakhapatnam, India


  • Research Article   
    Stability Indicating Method Development and Validation of Lamivudine, Zidovudine and Nevirapine by Using HPLC
    Author(s): Som Shankar Dubey* and Mahesh Duggirala

    A simple, efficient, and robust stability-indicating RP-HPLC method has been developed and validated to measure Lamivudine, Zidovudine and Nevirapine at a single wavelength (268 nm) in order to assay. The samples were eluted in an isocratic method using an inertsil ODS column (4.6 mm × 250 mm with a particle size of 5 μ) with a mobile phase consisting of 10 mM Ammonium acetate buffer (with a pH adjusted to 3.8 using Acetic acid): acetonitrile (60:40, v/v), acetonitrile and water (1:1, v/v) using as diluent, through ambient temperature delivered at a flow rate 1.2 mL/mins. A good linear response was obtained in the range from 15-75 μg/mL, 30-150 μg/mL and 20-100 μg/mL of Lamivudine, Zidovudine and Nevirapine respectively. The LODs for Lamivudine, Zidovudine and Nevirapine were found to be 0.315, 0.405 and 0.600 μg/mL, respectively and t.. Read More»

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