Der Pharma Chemica
Journal for Medicinal Chemistry, Pharmaceutical Chemistry, Pharmaceutical Sciences and Computational Chemistry

ISSN: 0975-413X


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Wael Talaat

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Damanhour University, Cairo, Egypt


  • Research   
    Comparative Chomatographic Study for Determination of Dalfampridine and its Derivative in Pharmaceutical Formulations
    Author(s): Khadiga M. Kelani, Ahmed M. Wafaa Nassar*, Wael Talaat and Samir Morshedy

    Two sensitive and precise methods were developed and validated for simultaneous estimation of dalfampridine with its oxidative degradation in pharmaceutical formulations without noticeable interference. Among the techniques adopted were chromatography [coupled TLC-densitometry and HPLC]. Method I: chromatographic separation a Spheri-5 RP C8 (220 X 4.6 X 5μm particle size) column was used in addition to the mobile phase [acetonitrile and 0.05 M KH2PO4 - (pH=5), (65: 35 v /v)], with a flow rate of (1ml.min−1) by detection (UV) at 298 nm. Method II: Densitometric separation of the drugs was performed on aluminium plates precoated with silica gel 60 F254 plates, with mobile phase chloroform, acetonitrile and methanol (volume 6:3:1) and measuring densitometry at 254 nm. HPLC calibration outline were set by the zone (0.2-6 μg mL-1), an.. Read More»

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